International Mammalian Genome Society

17th International Mouse Genome Conference

9-12 November 2003, Braunschweig, Germany


POSTER 58 - MOUSEEXPRESS: RNA EXPRESSION PROFILING OF ENU MOUSE MUTANTS

Seltmann M
GSF Research Center for Environment and Health, Experimental Genetics, Ingolstaedter Landstrasse 1, D-85764 Neuherberg/Muenchen

Co-Authors: 1) Horsch M, 1) Drobyshev A, 2) Mader M, 2) Tornow S, 3) Frohme M, 3) Korica T, 2) Mewes W, 3) Hoheisel J, 1) Hrabe de Angelis M, 1) Beckers J
Institutions: 1) GSF Research Center for Environment and Health, Experimental Genetics, Ingolstaedter Landstrasse 1, D-85764 Neuherberg/Muenchen. 2) GSF Research Center for Environment and Health, Bioinformatics, Ingolstaedter Landstrasse 1, D-85764 Neuherberg/Muenchen, 3) DKFZ, Functional Genome Analysis, Im Neuenheimer Feld 580, D-69120 Heidelberg

Comparative genome wide expression profiling is a powerful tool in the effort to annotate the mouse genome with biological function. The routine analysis of RNA expression data from organs of mouse mutant lines from the Munich ENU mutagenesis screen will reveal the molecular biology of such mutants and provide new insights into mammalian gene function.

Aiming for large scale and high throughput analysis we show in a direct comparison of pooled versus individual samples of organs that pools may be used as a highly efficient screening tool. In order to reduce biological noise of our system to a minimum we applied highly stringent housing and dissection conditions. We present data on wildtype individuals demonstrating the positive effect of controlling parameters such as social status, food intake before necropsy, and stress with regard to reproducibility of gene expression patterns. The results of an initial analysis of 10 organs each of 10 ENU mutant lines are summarised and discussed.


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