International Mammalian Genome Society

17th International Mouse Genome Conference

9-12 November 2003, Braunschweig, Germany


POSTER 63 - FINE AND DEEP HAPLOTYPE MAPPING OF CHROMOSOME 17 IN INBRED AND WILD MOUSE

Trachtulec Z
Institute of Molecular Genetics

Co-Authors: Vlcek C, Mihola O, Forejt J
Institutions: Institute of Molecular Genetics

We present a high-resolution haplotype map of a 241 kb region of chromosome 17, based on typing of 63 mouse chromosomes from five (sub)species and three t-haplotypes on seven SNP loci, two insertions/deletions, two microsatellites, and one minisatellite. The polymorphisms were initially defined by comparing the C57Bl/10SnPh and C3H/DiPh strains and are thus relevant for the positional cloning of the Hybrid sterility 1 (Hst1) gene. There are three types of haplotype blocks in classical inbred strains in this region and all three are derived from Mus m. domesticus. We found shorter haplotype blocks in some other laboratory strains and very short blocks in wild and wild-derived mice. Our results indicate that the megabase-sized blocks found previously in the classical strains are due to a limited number of chromosomes, which entered the inbredization process, rather than being a general feature of the mouse genome. Our map suggests the Hst1 alleles originated in the wild, which makes the Hst1 a good candidate for a speciation gene. Our data carry important implications for positional cloning projects in the mouse. It is possible to narrow down a candidate region by phenotyping and haplotype mapping on pre-selected strains more precisely than by a genetic analysis on a large cross of animals. The haplotype structure of the mouse genome makes the wild mouse a good model to study human haplotypes.


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