International Mammalian Genome Society

17th International Mouse Genome Conference

9-12 November 2003, Braunschweig, Germany


POSTER 78 - BARTMICE (BAYLOR, REPRODUCTIVE TRANSGENICS): A NATIONAL RESOURCE OF MICE, MUTANT IN GENES AFFECTING SEX DETERMINATION AND FERTILITY

Bishop CE
Baylor College of Medicine

Co-Authors: Poirier C, Overbeek P
Institutions: Baylor College of Medicine

We have recently initiated a large scale mutagenesis project using transgenesis and transposon mediated gene trapping. This is designed to generate an NIH funded, national resource of new mouse strains, mutant in genes affecting sex determination and fertility. The scheme is based on the rescue of albinism in the inbred FVB/N strain by the introduction a tyrosinase minigene, flanked by splice acceptors and Sleeping Beauty (SB) transposable elements. Mutants will be detected at two levels. Firstly, 200-300 founder transgenic families per year will be examined for fertility. Any insertional mutants will be identified and the integration sites FISH mapped, cloned and sequenced. Secondly, selected transgenic insertions, in particular those on the X and Y, will be mobilized using the SB transposase. Trasposon jumps will be identified by coat color changes and again the families tested for fertility. The insertion sites from such mutants can be rapidly cloned and sequenced by PCR.

To date we have identified five new transgenic families with defects in fertility, and three families showing embryonic lethality. Cloning the DNA flanking the transgenes is underway. In addition, we have identified one embryonic lethal, using transposon mediated jumping and cloned the insertion sites. Comparison with the mouse genome sequence shows that it integrated at MMU 6G1 in the second intron of a novel gene #5830457j20rik. An overview and update of this project will be presented. All data can be seen at www.mousegenome.bcm.tmc.edu/bartmice.


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