International Mammalian Genome Society

17th International Mouse Genome Conference

9-12 November 2003, Braunschweig, Germany


POSTER 79 - MOUSE SPERM AND DNA ARCHIVING – BUILDING A PLATFORM FOR GENOME RESEARCH

Boersma A
GSF-Institute for Experimental Genetics

Co-Authors: Marschall S, Peters D, Hrabe de Angelis M
Institutions: GSF-Institute for Experimental Genetics

During the last years a large number of new mouse mutant lines could be generated, e.g. via ENU mutagenesis. As these mutants represent a valuable resource as models for human diseases, it is extremely important to preserve all of these strains. For this purpose, at our institute a large-scale sperm and DNA archive has been established.

The animals to be archived (C3HeB/FeJ background) are mutants from an ENU mutagenesis screen and first generation (F1) males descending from ENU treated males. The sperm is frozen using French straws according to standard operation protocols which ensure consistent quality. Sperm quality is measured using a computerized semen analyzer. The rederivation is tested by performing an in vitro fertilization and embryo transfer. The laser microdissection of the zona pellucida is used for strains which cannot be recovered using conventional methods.

At present, the archive comprises 1440 ENU mutant males from 404 strains and 3488 F1 males.

Genomic DNA is extracted from tail clips and/or spleens for archiving. This allows screening the DNA for mutations in relevant genes. Having identified a DNA sample carrying a mutation, the mouse will be recovered from the frozen sperm archive for phenotypic analysis. Thus the archive provides a significant chance of recovering an allelic series of point mutations at any locus.

In conclusion, the sperm and DNA archive is a valuable platform for the development of models for human diseases.


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