International Mammalian Genome Society

17th International Mouse Genome Conference

9-12 November 2003, Braunschweig, Germany


POSTER 104 - RETINAL COLOBOMA IN FLS MICE

Matsuura T
Setsunan University

Co-Authors: 1) Kodama Y, 1) Ozaki K, 2) Hirasawa S, 1) Narama I
Institutions: 1) Setsunan University, 2) Shionogi & Co., Ltd.

A new type of morphological anomalies of the retina were detected in a new strain of mouse named FLS (fatty liver Shionogi), derived from the dd strain. In this study, eyes of FLS mice during lactation period were investigated morphologically. Animals postpartum days 7, 21 and 35 were fixed by perfusion of solution containing 4% paraformaldehyde and 2% glutaraldehyde. Then, both eyeballs were enucleated, and the cornea was removed along a horizontal line between the cornea and conjunctiva. Under a binocular stereoscope, the fundus was carefully observed, then they were dehydrated and embedded in paraffin, and subjected to histological examination by light microscopy. Macroscopic observation of the fundus revealed that incidence of retinal coloboma with retina fold was clearly high (more than 70 %) in males and females of FLS mice. Light microscopically, disarrangement or rosette formation of retinal layers was noted, and almost whole retinal layers disappeared in the area of macroscopically abnormal, accompanying with degenerated pigment epithelial cells. Furthermore, the retinal coloboma was always accompanied with invagination of ciliary body. From these results, FLS mice are considered to be a model for one of the inherited human retinopathy, retinal coloboma.


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