International Mammalian Genome Society

17th International Mouse Genome Conference

9-12 November 2003, Braunschweig, Germany


POSTER 162 - ANALYSIS OF THE GENETIC VARIANCE OF BODY WEIGHT IN CHROMOSOME SUBSTITUTION STRAINS OF THE MOUSE LINE DU6I

Brockmann GA
Research Institute for the Biology of Farm Animals, Dummerstorf, Germany

Co-Authors: Bevova M R, 1) Aksu S, 2) Aulchenko J, 1) Renne U
Institutions: 1) Research Institute for the Biology of Farm Animals, Dummerstorf, Germany, 2) Erasmus University of Rotterdam, Netherlands

The mouse line DU6i is a model for polygenic growth and obesity, which is an inbred derivate from the line DU6, selected over 100 generation for the high body weight at the age of 42 days. So far, many QTLs have been identified in the intercrosses DU6 x DUKs and DU6i x DBA/2 (Brockmann et al., 1998, 2000, 2001). Evidence was found that loci having small direct effect on growth or obesity contributed to the obese phenotype by gene-gene interaction. For more precise characterisation of effects of single QTLs and targeted analysis of gene-gene-interaction, we generated chromosome substitution strains (CSS) for DU6i mice. CSS were constructed by recurrent backcrossing of DU6i mice to the commercial inbred line DBA/2 as recipient line. The transfer of the chromosome of interest and the genetic background of the recipient line was controlled by 148 genetic markers. Here we present results on body weight obtained in the process of derivation of CSS, up to the sixth backcross generation. The weight of mice was accessed at the age of 21, 42, 56, and 63 days. Chromosomes 5, 7, 10, 12, 13 and 17 have shown significant effects on body weight. Different chromosomes were acting at different stages of mouse development. The biggest effect on body weight was found on chromosome 5; the effect was significant at all ages studied.


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