International Mammalian Genome Society

17th International Mouse Genome Conference

9-12 November 2003, Braunschweig, Germany


POSTER 168 - A SYNTHETIC MUTANT ON CHR 17 IS STERILE DUE TO LACK OF SPERM FLAGELLAE

Elliot R
Roswell Park Cancer Inst

Co-Authors: Tabaczynski D, Pazik J, Hohman C.
Institutions: Roswell Park Cancer Inst

Male F1 hybrids between inbred strains and M. macedonicus are sterile. To determine the genetic basis for this sterility, (C57BL/6J x M. macedonicus)F1 females were mated to C57BL/10J. A genome-wide scan with 92 SSLPs was performed for 141 male progeny of this cross and QTLs accounting for about 50% of the testis weight variance were identified on proximal Chrs 17 and X.

To confirm these findings, a congenic line, C57BL/6.MAC-17 has been constructed. The congenic interval extends from position 0 to position 23 on Chr 17 Congenic males have testes weighing about 0.15 gm, compared to weights of about 0.2 gm for the parents. Testis histology shows a lack of sperm tails. Tubules become plugged with sperm heads that are not released from Sertoli cells. Subcongenic males derived during the breeding have been homozygosed. Overlapping homozygous subcongenic strains identified by testis weight define an interval of about 2.5 Mbp. This interval contains 38 genes, including Dnahc8, a dynein involved in sperm flagellum formation, and associated with hybrid sterility 6, which is an excellent candidate. Supported by NIH grant GM33160.


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