International Mammalian Genome Society

17th International Mouse Genome Conference

9-12 November 2003, Braunschweig, Germany


POSTER 178 - 3-LOCUS SEGREGATION MODEL OF THE LEYDIG CELL RESPONSIVENESS DURING THE POSTNATAL DEVELOPMENT IN MICE: MULTIGENIC AND MULTIVARIABLE APPROACH

Osadchuk A V
Institute Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences

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Aim of the study was to develop an optimal segregation model describing heritable variation in the Leydig cells responsiveness (LCR) during the postnatal development in diallel cross of four inbred mouse strains (CBA/Lac, A/He, BALB/c, PT) contrasting in adult LCR pattern. The LCR was estimated by testosterone production by crude Leydig cell suspension in response to submaximal concentrations of hCG, d,b-cAMP and pregnenolone on days 20, 35, 60 and 100 of the postnatal life in all 16 crosses. To detect the structure of genetic relationships between the traits, factor analysis of the among-cross correlation matrix was performed. It produced a single factor that expressed the coordinated LCR and accounted for 80% of the total among-cross variance for each time-point of the postnatal development. Based on multiple regression analysis of the diallel matrix and subsequent enumeration of all possible genotype variants in the parental strains, we developed the simplest 3-locus segregation genetic model that adequately described of among-cross variation relative to the within–cross environmental noise. This model accounted for about 85% of genotype-dependent among-cross variation in every time-point of the postnatal development. The model included two autosomal loci with biallelic expression and one locus with paternal monoallelic expression. In conclusion, the complexity of the LCR heritable pattern can be explained by substantial changes of main and epistatic genetic effects of these loci during the postnatal development. The approach can be used for genetic dissection of complex traits during ontogenesis. The work was supported by the grant from RFBR 01-04-49523.


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