International Mammalian Genome Society

17th International Mouse Genome Conference

9-12 November 2003, Braunschweig, Germany


Platzer M
Institut für Molekulare Biotechnologie (IMB) Jena

Co-Authors: 2) Monti J, 1) Tänzer S, 3) Scharfe M, 2) Gösele C, 1) Glöckner G, 1) Galgoczy P, 2) Liska F, 3) Blöcker H, 2) Hübner N
Institutions: 1) Institut für Molekulare Biotechnologie (IMB) Jena 2) Max-Delbrück-Centrum für Molekulare Medizin (MDC) Berlin-Buch 3) Gesellschaft für Biotechnologische Forschung (GBF) Braunschweig

The rat is a major animal model for experimental investigations of complex diseases. Rat strains reflecting human complex diseases have become an important reductionist tool and have been used for the identification of multiple QTL. So far a large number of disease-associated QTL has been identified in co-segregation studies of rat intercrosses. Clusters of overlapping disease loci were identified on rat chromosomes 1 and 10, determining especially cardiovascular phenotypes like hypertension, renal failure, diabetes, and stroke.

Alignment of the rat genomic sequence with maps from human and mouse enables the construction of comparative maps and the consideration of candidate genes in defined intervals. Ultimately high quality sequence will be required in the relevant QTL region. Therefore bacterial clones were mapped to the respective QTLs. In the initial sequencing phase BACs were end sequenced. Following, based on selected clones a working draft sequences of 8x coverage was generated, making use of the publicly available rat genome sample sequences. Finally, BACs containing positional or functional candidate genes were finished to high quality standard, analyzed and annotated.

This effort is closely coordinated with the US project which provided a working draft of the rat genome. At the conference, the current stage of mapping, sequencing and comparative genome analysis of QTLs for cardiovascular phenotypes on rat chromosome 1 and 10 will be presented.

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