International Mammalian Genome Society

17th International Mouse Genome Conference

9-12 November 2003, Braunschweig, Germany


ORAL PRESENTATION

WEDNESDAY 12 NOVEMBER
14:45 – 15:00 HRS

INTEGRATIVE TRANSOMIC ANALYSIS STRATEGIES REVEAL NOVEL INSIGHTS INTO POTENTIALLY IMPORTANT MOLECULAR PATHWAYS FOR MAMMARY TUMOR METASTASIS

Hunter K
Laboratory of Population Genetics, NCI, NIH

Co-Authors: Park Y-G, Yang H, Zhang J, Lukes L, Lancaster M, Buetow K
Institutions: Laboratory of Population Genetics, NCI, NIH

Our laboratory has demonstrated that the genetic background of tumor significantly influences the ability of the tumor to disseminate. Using conventional QTL mapping crosses and RI strains, we demonstrated the presence of at least 5 genomic regions that were significantly or suggestively associated with metastatic efficiency. Conventional congenic mapping experiments are currently in progress to achieve high resolution mapping of candidate loci. In parallel, to supplement and accelerate the candidate gene identification process, we have been performing an integrative, trans-disciplinary strategy utilizing a variety of “-omic” technologies to develop a more comprehensive pathway-based understanding of the metastatic process. Combining genetic, somatic cell genomics, bioinformatics, haplotype mapping, microarray analysis, proteomics and genome-wide transcriptional QTL mapping, we have identified a molecular process that plays an important role in metastatic progression. The identification of this pathway as a potentially important element in tumor dissemination has recently been supported by the confirmation that the murine ortholog of the breast cancer metastasis suppressor gene Brms1 is an integral member of the complex. Additionally, this complex is potential at the nexus of a number of metastasis associated genes. Finally, all of our genetically defined QTL peaks contain members of this molecular process, suggesting that they may be interesting candidate genes. Our data to date suggest that integrative “transomic” strategies may provide a vehicle to significantly accelerate QTL gene identification and analysis. In vitro molecular and in vivo complementation assays are currently in progress to evaluate the significance of our transomic findings.


Abstracts * Officers * Bylaws * Application Form * Meeting Calendar * Contact Information * Home * Resources * News and Views * Membership

Base url http://imgs.org
Last modified: Saturday, November 3, 2012