International Mammalian Genome Society

17th International Mouse Genome Conference

9-12 November 2003, Braunschweig, Germany


POSTER 10 - KERATIN 14 CRE MICE PROVE MATERNAL OOCYTIC KERATIN 14 EXPRESSION

Hafner M
German Research Cente for Biotechnology

Co-Authors: 2) Haase I, 3) Scharfetter-Kochaneck K, 4) Pasparakis M, 1) Schippers A, 2) Krieg T,1) Müller W
Institutions: 1) German Research Centre for Biotechnology, 2) University of Cologne, 3) University of Ulm, 4) EMBL, Monterotondo

While displaying tissue specific Cre-mediated deletion in the epidermis, tongue and thymic epithelium when inherited from the father, three independently generated Keratin 14 Cre (K14Cre) mouse lines display complete deletion of loxP flanked DNA segments in the offspring if the mother carried the transgene. Complete deletion is even observed in littermates that lack the K14Cre allele due to transmission of the transgene to the polar bodies during oogenesis. Thus, maternally expressed Cre protein remains active within the oocyte until after fertilisation. The oocytic K14Cre activity was further proven by reporter gene activation in maturing oocytes in preantral and antral follicles. Since Keratin 14 (K14) mRNA expression could be identified by PCR in ovaries and K14 could be detected in oocytes of preantral and antral follicles it can be concluded that K14 is an authentic oocytic protein.


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