International Mammalian Genome Society

17th International Mouse Genome Conference

9-12 November 2003, Braunschweig, Germany


POSTER 37 - CHEMICAL MUTAGENESIS OF MOUSE EMBRYONIC STEM CELLS

Himmelbauer H
Max-Planck-Institute of molecular Genetics, Department of Vertebrate Genomics, Berlin-Dahlem, Germany

Co-Authors: Greber B, Lehrach H
Institutions: Max-Planck-Institute of molecular Genetics, Department of Vertebrate Genomics, Berlin-Dahlem, Germany

Despite the availability of mammalian genome sequences, the functions of many genes remain unknown. Mutant mice play an important role to study mammalian gene functions. It would therefore be desirable to ultimately create a mutant for every gene in the mouse genome. Most probably, different complementary approaches will be required to achieve this goal.

Embryonic stem cell technology offers the possibility to manipulate the mouse genome and select for mutations in vitro. Mutant animals can then be generated from selected clones. We are inducing mutations chemically using ENU, followed by the generation of mutant clone libraries in a 96-well format. Such clone libraries can be specifically screened for mutations in genes of interest. We are applying a protocol that allows the effective detection of mutations based on pooled cDNA samples. Methodology and screening results will be presented at the meeting.

Acknowledgement of support: Our work is carried out through funding by the German National Genome Research Network (NGFN).


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