International Mammalian Genome Society

17th International Mouse Genome Conference

9-12 November 2003, Braunschweig, Germany


POSTER 38 - COMMON AND DISTINCT EXPRESSION PATTERNS OF THE IGF-SYSTEM IN MICE IN RESPONSE TO DIVERGENT GROWTH SELECTION

Hoeflich A
Institute of Molecular Animal Breeding and Biotechnology University of Munich

Co-Authors: 2) Bünger L , 1) Nedbal S, 3) Renne U, 4) Elmlinger M W, 5) Blum W F, 2) Bruley C, 6) Kolb H J, 1) Wolf E
Institutions: 1) Institute of Molecular Animal Breeding and Biotechnology University of Munich 2) Institute of Cell, Animal and Population Biology, University of Edinburgh 3) FBN Dummerstorf 4) Institute of Endocrinology University of Tübingen, 5) Lilly Deutschland Bad Homburg, 6) Institute of Clinical Chemistry Munich

Transgenic and knockout models have been used successfully in order to attribute specific functions to distinct growth factors. Here we ask how in a complex organism expression of growth factors in fact is affected during altered growth: are there general patterns of growth control which are found in different models or is growth control achieved by unique combinations of different expression levels of different growth factors? These questions can ideally be answered by employment of mouse models generated via the phenotype-driven approach through long-term selection for high or low body weight. Lines of mice selected for many generations for high (H) or low (L) growth in different laboratories have been collected. We have studied systemic and local expression of growth relevant genes in 8 of these mouse lines highly diverging for body and carcass weights but also for nose-rump lengths. Serum IGF-I levels were severely reduced in all L-lines if compared to unselected controls, whereas the serum IGF-I levels were elevated only in one H-line. In no case we were able to identify a reduction of the IGFBPs present in the circulation. Instead, IGFBP-3 was elevated exclusively in distinct H-lines, while IGFBP-2 was the only IGFBP elevated in different L-lines, identifying IGFBP-2 as an exclusive negative effector for growth in the circulation beyond all other IGFBPs. In muscle tissue from selected breeding groups characterized by specific increases of the carcass weights we found different patterns of gene expression and describe highly coordinated gene expression of growth relevant genes.


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