International Mammalian Genome Society

17th International Mouse Genome Conference

9-12 November 2003, Braunschweig, Germany


POSTER 44 - TOWARDS A COMPARATIVE PROTEOME/TRANSCRIPTOME ANALYSIS OF GENES EXPRESSED IN LIVER AND KIDNEY OF THE MOUSE

Mijalski T, Drobyshev A, Horsch M, Schädler S
Institute of Experimental Genetics, GSF - National Research Centre for Environment and Health, Munich, Germany

Co-Authors: 2) Halder T, 2) Harder A, 3) Lottspeich L, 1) Hrabe de Angelis M, 1) JBeckers B
Institutions: 1) Institute of Experimental Genetics, GSF - National Research Centre for Environment and Health, Munich, Germany, 2) TopLab GmbH, Martinsried, Germany, 3) Max-Planck Institute for Biochemistry, Martinsried, Germany

So far, little is known about the relationship between the transcriptional and post-transcriptional regulation of gene expression in complex organisms. Comparative transcriptome and proteome analyses in the mouse as mammalian model organism have not been performed. We use DNA-chip based RNA expression profiling and 2D-gelelectrophoresis (in collaboration with T.H. and A.H.) to explore optimal conditions and the general feasibility of such comprehensive gene expression analyses. To establish and optimise comparative gene expression techniques we made an assessment of RNA and protein profiles of adult liver and kidney in the mouse. These tissues can be obtained as recoverable biological resource under reproducible conditions from inbred mouse strains. We provide one of the most comprehensive data sets on differential gene expression in both organs on the RNA and protein level.

We identified several hundred differentially expressed genes that were analysed for their correlation on the RNA and protein level, their correlation between individual mice, and for their chromosomal location. Besides genes with known functions, we also identified a significant number of novel genes without any functional annotation, so far.


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