International Mammalian Genome Society

17th International Mouse Genome Conference

9-12 November 2003, Braunschweig, Germany


POSTER 45 - EMBRYONIC EXPRESSION OF Raidd USING β -galREPORTER GENE IN TRANSGENIC MICE

Motaln H
University of Ljubljana, Biotechnical faculty, Department of Animal Science, Groblje 3, 1230 Domzale, Slovenia

Co-Authors: 2) McWhir J, 1) Horvat S
Institutions: 1) University of Ljubljana, Biotechnical faculty, Department of Animal Science, Groblje 3, 1230 Domzale, Slovenia, 2) Roslin Institute, Division of Gene expression and Development, EH25 9PS Roslin, Midlothian, Scotland, UK

Apoptosis and differentiation are tightly intertwined processes occurring at organ formation and remodelling during embryonic development. Raidd, a dual-domain adaptor protein, has been shown to mediate the recruitment of Caspase-2 to tumour necrosis factor receptor 1 (TNFR-1) signalling complex through RIP kinase. So far, Raidd transcripts of 1,4 kb and 1,8 kb have been detected by Northern analysis in whole embryos at some stages, in the foetal liver, and in most adult tissues, but a detailed analysis of the tissue-specific pattern of expression has not yet been reported. Raidd overexpression studies suggest that apart from the established role in the cascade of apoptotic pathway, Raidd may have additional function during development. To elucidate the role of Raidd during mouse embryogenesis, we examined Raidd expression pattern in midgestation mouse embryos where the processes of organogenesis are most dynamic. In the study, we utilized our previously established Raidd+/- mouse line carrying a reporter transgene (Beta-galactosidase, β -gal) under the control of Raidd promoter. This study provides the first in situ analysis of Raidd expression in different tissues of embryonic stages E8.5 - E12.5 performed by histochemical staining of whole embryos. Expression analysis revealed that the spatial change in Raidd expression is stage and tissue/organ specific. Furthermore, increased expression of Raidd seemed to be confined to the areas undergoing dynamic morphological changes. Therefore, the associations between staining and early processes of organ formation/remodelling activity suggest that Raidd could play a physiologically important role in organogenesis during embryonic development in mice.


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