International Mammalian Genome Society

17th International Mouse Genome Conference

9-12 November 2003, Braunschweig, Germany


Nagaraja R
National Institute on Aging, NIH, Baltimore, U.S.A

Co-Authors: 1) Brathwaite M, 1) Waeltz P, 1) Schroeder M, 1) Jefferson J, 2) Schein J, 2) Marra M, 3) Abe K and 4) Roe B
Institutions: 1) National Institute on Aging, NIH, Baltimore, U.S.A, 2) Canada's Michael Smith Genome Science Center, Vancouver BC, Canada, 3) Riken Tsukuba Institute, Ibaraki 305-0074, Japan, 4) Advanced Center for Genome Technology, University of Oklahoma, Oklahoma, U.S.A.

Many embryonic lethal mutations and genes associated with stemness and growth control fall in the “t-complex” on mouse chromosome 17. The challenge of analyzing this region and identifying genes bearing lethal mutations is increased because of the duplications and four large segments that undergo inversion. To facilitate the analysis, in conjunction with mouse genome sequencing efforts, a BAC/STS physical map was assembled in large portions of the region, and 43 BACs were sequenced. Sixteen of the estimated 30 Mb region has been assembled in 3 contigs and integrates BAC clones, recovered by screening with STSs; and supported by fingerprint data. The information confirms much of the genome sequence assembled from other sources, and adds coverage of segments not yet recovered. Contig 1, ~8.0 Mb, extending from MGD cM 4.0 to 8.3, with 188 BACs formatted with 166 STSs, includes markers Brachyury at the proximal end and D17Leh55 distally, and covers the distal border of inversion 2, where other sequence coverage is poor. Contig 2, 7.3 Mb, spans MGD cM 8.8 to 16.4, and consists of 177 BACs and 157 STSs. Contig 3, 1Mb proximal to H2-K, defines the recessive lethal tw5 critical region, includes 13 BACs and 19 STSs, and precisely localizes markers D17Mit147, D17Mit16, and D17Mit62. Sequenced clones were annotated, yielding 7.97 Mb sequence that independently verify and add to whole genome shotgun assembly, and providing candidate genes and clones to investigate tw5 and other t-complex lethal mutations.

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