International Mammalian Genome Society

logo18th International Mouse Genome Conference

17-22 October 2004, Seattle, USA


Takahashi A 1, Takahashi A 4, Yonekawa H 2, Shiroishi T 1, Shiroishi T 3, Koide T 1, Koide T 4

1 The Graduate University of Advanced Studies, Mishima, Japan, 2 The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan, 3 Mammalian Genetics Laboratory, National Institute of Genetics, Mishima, Japan, 4 Mouse Genomics Resource Laboratory, National Institute of Genetics, Mishima, Japan

Open-field is one of the widely used methods for measuring emotionality. Recently, genetic mapping studies have reported some QTLs for open-field behavior. Most of them used two major variables, ambulation and defecation, that totaled per test. However, when a mouse is placed into the open-field, they show a variety of behavior such as scout and rear to look around, or groom their body. Also, frequency of those behaviors changes with time as the mouse accustomed to the environment. In this point of view, detailed analysis of the open-field behavior is important to understand psychological status of the mice. The focus of the present work was to investigate temporal analysis of the detail open-field behavior.

The wild mouse strains were established as inbred strains from wild mice. These strains are known to have a large inter-strain genetic diversity, because they were captured from many countries. In the present study, we demonstrated the descriptive analysis of the open-field behavior by scoring 12 component of the behavior on 10 wild mouse strains, a laboratory mouse strain C57BL/6, and a Japanese fancy mouse strain JF1. They showed a great diversity in the frequencies and the temporal changes of those open-field behaviors. The pattern of the behaviors was much more complicated than which were shown in ambulation or defecation. We next performed this detailed analysis of the open-field behavior on consomic mouse strains that were composed of wild MSM and laboratory C57BL/6. We found some cosomic strains showing a significant difference in the detailed open-field behaviors.

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