International Mammalian Genome Society

logo18th International Mouse Genome Conference

17-22 October 2004, Seattle, USA


POSTER 65 - MICROARRAY ANALYSIS OF GENE EXPRESSION AT THE DEL(13)SVEA36H REGION OF MOUSE CHROMOSOME 13 DURING POSTIMPLANTATION MOUSE DEVELOPMENT

Willoughby C 1, Davies J 1, Underhill P 1, Holmes C 1, Greenfield A 1, Denny P 1, Ragoussis J 2, Arkell R 1

1 Mammalian Genetics Unit, MRC Harwell,, Oxfordshire, United Kingdom, 2 Wellcome Trust Centre for Human Genetics, Oxford, United Kingdom

Radiation induced chromosomal deletions in mouse have been used in the functional analysis of defined regions in a number of studies. Within the Mammalian Genetics Unit at MRC Harwell attention has focused on the annotation of the Del(13)Svea36H deletion on chromosome 13. This annotation has involved the sequencing and comparative sequence analysis of the region, in parallel with a screen for recessive ENU-induced mutations at the region. The screen has identified 11 embryonic lethal mutations and as part of the ongoing analysis we aim to clone the mutated genes. In this type of cloning experiment a key stage is the identification of the expression pattern of genes within the critical region. We have decided to utilise microarray technology to assist in this positional candidate approach to identification of these mutations. We have used whole genome spotted oligo arrays to create an expression profile of the genes in the Del(13)Svea36H deletion between 6.0 days post coitum (dpc) and birth. Since the experiment utilises whole genome mouse arrays it also constitutes a profile of postimplantation mouse development which has not previously been undertaken.

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