International Mammalian Genome Society

logo18th International Mouse Genome Conference

17-22 October 2004, Seattle, USA


POSTER 69 - STUDY OF THE GENES INVOLVED IN THE HYPERTROPIC LANGERHANS ISLAND OF THE KK-AY MOUSE AND THEIR HISTOLOGICAL LOCALIZATION

Kusakabe M 1, Matsuba K 2, Inoue J 2, Aotsuka S 2, Hokao R 1, Hashimoto H 3

1 Institute for Animal Reproduction, Ibaraki, Japan, 2 ANB Tsukuba Institute, Aloka Co., Ltd., Ibaraki, Japan, 3 Jikei Univ. Sch of Med., Dept. Anat., Tokyo, Japan

A KK-Ay mouse is the experimental animal model for type II diabetes. Interestingly, it was found that the Langerhans Island of KK-Ay becomes hypertropic with age. Therefore, the purpose of this experiment is to search the genes, which are involved in the hypertropic Island, and to examine the histological localization of these genes.

The two-way subtraction libraries were made between the pancreas of BALB/cA, 10 week-old, and one of KK-Ay, 10 week-old.  After cloning of these libraries, we picked up at lease 1200 clones from each library and arrayed them on the membrane filters. These filters were then hybridized with the probes prepared from cDNA of each pancreas. The characteristic clones were selected and sequenced. Then, homology and cluster analysis were performed. Next, PCR analysis was done to select the clones, which are different in amount between diabetes and control. Finally, the probes for in situ hybridization were made from the selected clones.  We selected 162 clones from KK-BALB library (K-B) and 153 from BALB-KK library (B-K) as specifically expressed clones. After homology and cluster analysis, 28 genes in K-B and 24 in B-K were identified. Two unknown genes in K-B and 6 in B-K were also detected. After pcr, 9 in K-B and 10 in B-K were identified as more specific genes. Reg I, Reg II and Reg III beta, which are involved in regeneration, were specifically enhanced their expression in the KK-Ay mouse. At present, in situ hybridization to know histological localization of these genes is under way.

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