International Mammalian Genome Society

logo18th International Mouse Genome Conference

17-22 October 2004, Seattle, USA


POSTER 84 - THE MOUSE PANCCHIP: HIGH THROUGHPUT GENE EXPRESSION ANALYSIS OF THE ENDOCRINE PANCREAS

Mazzarelli J 1, White P 1, Gorski R 1, Brestelli J 1, Arsenlis A 1, Manduchi E 1, Katokhin A 2, Belova O 2, Bogdanova V 2, Elisafenko E 2, Gubina M 2, Nizolenko L 2, Perelman P 2, Puzakov M 2, Shilov A 2, Trifonoff V 2, Vorobjeva N 2, Voronin D 2, Zykov I 2, Kolchanov N 2, Kaestner K 1, Stoeckert C 1

1 University of Pennsylvania, Philadelphia, United States, 2 Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia

The mouse pancreas-enriched microarray or mouse PancChip, provides a powerful tool for studying pancreatic development and diabetes research, allowing the investigation of gene expression for over 13,000 transcripts expressed in the pancreas. In designing the PancChip, one clone from mouse pancreas libraries was picked to represent each unique mouse transcript as identified by computational analysis using the Database of Transcribed Sequences (DoTS).

Computational and manual annotation of the transcripts has identified known and novel transcripts and their functions.  Using the PancChip, we show that these transcripts are expressed in purified islets and the pancreas.  The novel transcripts define new mouse genes when BLAT-aligned to the mouse genome.  Genomic alignment of the transcripts also identifies alternative transcripts of known genes that are expressed in the pancreas.  Interestingly, some transcripts that are expressed are non-coding RNAs and overlap microRNA genes aligned to the genome. 

Information regarding the mouse PancChip and the transcript annotation is accessible via the web site of the Endocrine Pancreas Consortium, EPConDB ( http://www.cbil.upenn.edu/EPConDB/index.shtml).

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