International Mammalian Genome Society

logo18th International Mouse Genome Conference

17-22 October 2004, Seattle, USA


POSTER 135 - NINE STRAIN-SPECIFIC MOUSE BAC LIBRARIES

Nefedov M 1, Zhu B 1, Yoshinaga Y 1, Luo M 2, Wing R 2, Tomkins J 3, Misceo D 1, Hallers B 1, Cao Q 1, Osoegawa K 1, De Jong P 1

1 BACPAC Resources, Children’s Hospital Oakland Research Institute, Oakland, CA94609, United States,

2 Arizona Genomics Institute, Tucson, AZ 85721, United States, 3 Clemson University Genomics Institute, Clemson, SC 29634, United States

With the release of the human and mouse draft DNA sequences, there is a growing need for functional evaluation of various genes and their regulatory elements.  We constructed nine 10-fold redundant mouse strain-specific BAC genomic libraries from inbred mouse strains: Mus musculus C57BL/6J, DBA/2J, NOD, A/J, BALB/cByJ, C3H/HeJ, AKR/J, and feral strains derived from M. m. castaneus and Mus spretus. Providing more than 10 genome equivalents, each library contains 160,000-200,000 clones with average 143-185Kb insert sizes, arrayed in 384-well plates. For screening, BAC clones were gridded onto high density nylon filters. The complete filter sets were screened by hybridization with 30 conserved overgo probes. The average number of about nine hybridization-positive clones per probe is in agreement with the overall redundancy of the libraries. Half of NOD mouse library (98,000 clones) will be end-sequenced to create a mapped BAC resource. These libraries are indispensable tools for the characterization of genes that contribute to complex traits and strain-specific phenotypes.

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