International Mammalian Genome Society

logo18th International Mouse Genome Conference

17-22 October 2004, Seattle, USA


POSTER 140 - Establishing  novel mutant mouse models that mimic human diseases through ENU-based genome-wide mouse mutagenesis

Kung JT

Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan 11529

With the human genome sequenced, the race is on to understand the functions of the nearly 40,000 or so genes. Under the support of the National Science Council and Academia Sinica, a national program of large-scale genome-wide mutagenesis of the mouse has been set up.  Ethyl-nitrosourea (enu) was used to treat founder C57BL/6 (B6) male mice, followed by a 3-generation breeding scheme to generate G3 mice that were screened for recessive phenotypes.  Two screening programs have been set up.  As a service to the research community, the G3 mice are provided as a service to investigators who has received funding to perform their unique phenotypic screens.  The core facility also performs phenotypic screens as part of its internal program.  So far, the core has been screening for blood cell composition, some blood chemistry assays and easily observed traits.  Mutants that have been identified include elevated platelet count, depressed white blood cell count, abnormal limb development, coat color mutant, etc.  Screening results and subsequent heritability testing results of these mutants will be presented and discussed.  Individuals with interests in performing phenotypic screens in a collaborative arrangement are welcome to visit our website at http://mmp.sinica.edu.tw or contact us at mouse@gate.sinica.edu.tw

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