International Mammalian Genome Society

logo18th International Mouse Genome Conference

17-22 October 2004, Seattle, USA


POSTER 156 - AUTOMATED DNA PURIFICATION FROM MOUSE TAILS

Mulrooney C, Howe S, Robinson J, Oultram J, Sayle J

Tepnel Life Sciences, Manchester, United Kingdom

The extraction of high quality genomic DNA is the first and often limiting step in the process of mouse tail genotyping.  Tail snips are difficult samples to work with and most of the chemistries and systems available today fail to consistently produce sufficient DNA of an appropriate quality to meet the requirements of PCR and Southern Blotting.

Tepnel’s Nucleopure system not only produces excellent yields of highly intact and pure DNA from 192 tail snips in less than 2 ½ hours, but can also set up downstream PCR reactions as well.

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