International Mammalian Genome Society

logo18th International Mouse Genome Conference

17-22 October 2004, Seattle, USA


POSTER 160 - IDENITIFICATION OF ENU-GENERATED MUTANTS FOR ANXIETY AND ACTIVITY IN AN OPEN FIELD TEST

Pletcher MT, Grabowski L, Wiltshire T, Tarantino L

The Genomics Institute of the Novartis Research Foundation, San Diego, United States

The determination of the complex genetic interactions that control behavioral phenotypes has been slow to progress.  In an attempt to exploit new technologies to unravel aspects of behavioral genetics, a forward genetics screen was established to identify phenotypic outliers in an open field test from a population of offspring from ENU-mutagenized mice.  Values were collected for percent time spent in the center of the open field, total distance traveled, percent time resting, amounts of continuous motion, average velocity, and total rearing.  Percent time spent in the center of the open field and average velocity show good correlation as a marker of anxiety in the mice while total distance traveled, percent time resting, and amounts of continuous motion track potential hyperactive phenotypes.  456 ENU-mutagenized families have been screened by this method with 30 being identified as outliers for anxiety and 19 having abnormal activity levels.  To date, 4 have been found to be heritable for one of the phenotypes screened.  The two lines that have been characterized most extensively are Frantic and Angry MouseFrantic shows an anxious-like phenotype of hyperactivity in novel environments while Angry Mouse is characterized by consistently increased activity level and aggressive behavior.  Currently, the causative mutations for these mouse lines are actively being mapped.

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