International Mammalian Genome Society

logo18th International Mouse Genome Conference

17-22 October 2004, Seattle, USA


POSTER 181 - THE PHENOTYPE OF AKT1, AKT2 AND AKT3 SINGLE AND DOUBLE KNOCKOUT MICE

Chen W, Hay N

1 University of Illinois at Chicago, Chicago, United Kingdom, 2 University of Illinois at Chicago, Chicago, United States

Akt, also known as protein kinase B (PKB), belongs to a serine/threonine kinase subfamily.  So far, three main isoforms of Akt have been identified in mammalian cells: Akt1, Akt2 and Akt3 encoded by 3 separate genes.  Studies have shown that Akt in cells is activated by diverse stimuli, such as hormones, growth factors and extracellular matrix components, and is a downstream target for phosphoinositide 3-kinase (PI3K).  It appears that Akt lies at the crossroads of multiple cellular signaling pathways, since activated Akt phosphorylates and regulates many cellular proteins involved in processes that include apoptosis, glucose metabolism, proliferation, transcription and angiogenesis.  In order to elucidate the physiological role of Akt1, Akt2 and Akt3, we use knockout mice as the model to define the function of Akt1, Akt2 and Akt3.  We will describe the phenotypes of individual and compound knockouts of the 3 Akt isoforms.

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