International Mammalian Genome Society

logo18th International Mouse Genome Conference

17-22 October 2004, Seattle, USA


ORAL PRESENTATION

TUESDAY OCTOBER 19

9.30am – 9.45am

IDENTIFICATION OF REGION-SPECIFIC TRANSCRIPTION FACTOR GENES IN THE ADULT MOUSE BRAIN BY MEDIUM-SCALE REAL-TIME RT-PCR

Suzuki H, Okunishi R, Hashizume W, Katayama S, Ninomiya N, Osato N, Sato K, Nakamura M, Iida J, Kanamori M, Hayashizaki Y

RIKEN GSC, Yokohama, Japan

Many transcription factors play important roles in the development and functions of the brain, although their low levels of expression in the brain’s complex tissues have hindered the exploration of their expression properties by DNA microarray technology. We established a medium-scale real-time RT-PCR system focusing on mouse transcription factors and applied it to their expression profiles in 11 regions of the adult mouse brain. Of a total of 756 transcription factor genes examined, 677 (89.6%) showed significant expression in at least one brain region, reflecting the high level of complexity of the mouse brain. We successfully extracted 179 (23.7%) region-specific genes by clustering analysis; they were observed in abundance in the medulla oblongata, cerebellum, pituitary gland and midbrain, but less so in the posterior cerebral cortex and hippocampus. Interestingly, most of the transcription factors involved in the development of the anterior pituitary were still expressed specifically in the adult pituitary, suggesting that these transcription factors play important roles in both development and maintenance of the pituitary. In addition to providing reliable expression profiles of transcription factors and a unique set of molecular markers for 11 brain regions, these results can help us to identify transcription regulation pathways that may account for the molecular basis of the unique functions of specific regions of the brain.

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