International Mammalian Genome Society

logo18th International Mouse Genome Conference

17-22 October 2004, Seattle, USA


POSTER 15 - THE EMAGE 3D GENE EXPRESSION DATABASE

Christiansen JH, Richardson L, Venkataraman S, Burton N, Yang Y, Baldock R, Davidson D

MRC Human Genetics Unit, Edinburgh, U nited Kingdom

The Edinburgh Mouse Atlas of Gene Expression (EMAGE) is a database of spatially mapped gene expression data in the developing mouse embryo that we are developing as part of the Edinburgh Mouse Atlas Project (EMAP).  EMAGE is part of the Mouse Gene Expression Information Resource (MGEIR) which is being built in a collaborative effort with the GXD/MGI group at the Jackson Laboratory.

All EMAGE data is housed in a standard framework: the EMAP Digital Atlas of Mouse Development which consists of at least one representative 3D digital embryo model for most Theiler stages (TS) as well as a standardised nomenclature for anatomical structures that are present at every Theiler stage of development.  As the digital models are 3D, it is possible to cut virtual sections in any plane through them to reveal anatomical detail within that plane.

Incoming EMAGE data images are mapped spatially into the corresponding  regions within the embryo models.  At TS07-14 the 3D standard models have anatomical regions defined within them and as such, data spatially mapped into these models is automatically annotated to the corresponding text terms for these structures.  This is accompanied by further manual text annotation.

Searching the data in EMAGE can be done spatially by a simple point and click method, or by using text terms. 

New developments aimed at identifying groups of co-expressed genes (by heirarchical clustering and domain intersection analysis) will be discussed.

EMAGE is publicly accessible at http://genex.hgu.mrc.ac.uk.

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