International Mammalian Genome Society

logo18th International Mouse Genome Conference

17-22 October 2004, Seattle, USA


POSTER 19 - COMPREHENSIVE SAGE ATLAS OF MURINE GENE EXPRESSION THROUGHOUT DEVELOPMENT

Bohacec S 1, Xie Y 1, Kuo B 1, Khattra J 2, Sidiqqui A 2, Helgason CD 4, Hoodless PA 3, Jones S 2, Marra M 2, Simpson EM 1

1 Centre for Molecular Medicine and Therapeutics, Vancouver, Canada, 2 Genome Sciences Centre, Vancouver, Canada, 3 Terry Fox Laboratory, B.C. Cancer Agency, Vancouver, Canada, 4 Cancer Endocrinology, B.C. Cancer Agency, Vancouver, Canada

This project is designed to generate a comprehensive collection of gene expression profiles encompassing various tissues throughout development, from fertilized egg to adult, for the C57BL/6J mouse. The use of mouse as a model of human development enables the systematic collection of embryonic tissues not possible in humans. Detection and quantitation of transcripts is carried out via the Long Serial Analysis of Gene Expression (LongSAGE) technique. This platform provides three major advantages over conventional gene expression analysis approaches: it presents a quantitative profile of the mouse transcriptome, it is not restricted to only detecting known genes, and it can be subsequently expanded as further data becomes available. To date, data sets from 44 libraries, of a planned 200, are publicly available. These libraries total over 5 million sequenced LongSAGE tags and represent manual or laser capture microdissected (LCM) tissues at significant stages in embryogenesis and organogenesis. A third of the projected libraries will profile gene expression in developing neural tissue with the aim of better understanding brain formation and a range of neural disorders. Two software packages (DiscoverySpace and CMOST) have also been developed for use in library analysis, inter-library comparisons and tag-to-gene mapping through numerous databases.

The main objective of the Mouse Atlas Project is to create a publicly accessible on-line expression database that will offer the research community insights into the progression and regulation of mammalian development for more rapid identification of disease genes and targets for new therapeutics. Library descriptions and full data sets are available on our website: http://www.mouseatlas.org/.

This project is supported by Genome Canada, the BC Cancer Agency and NCI-NIH.

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