International Mammalian Genome Society

logo18th International Mouse Genome Conference

17-22 October 2004, Seattle, USA


PLENARY PRESENTATION

TUESDAY OCTOBER 19

5.45pm – 6.15pm

IDENTIFYING COMPONENTS OF GROWTH FACTOR SIGNALLING PATHWAYS THROUGH GENE TRAP MUTAGENESIS

Soriano P

Fred Hutchinson Cancer Research Center, Seattle, United States

We have developed a versatile, high throughput genetic screening strategy by coupling gene mutagenesis and expression profiling technologies.  Using a retroviral gene trap vector optimized for efficient mutagenesis and cloning, genes were randomly disrupted in mouse embryonic stem cells and amplified to construct a cDNA microarray.  With this gene trap array, we demonstrate that PDGF transcriptional target genes can be efficiently and reliably identified in physiologically relevant cells, and are immediately accessible to genetic studies to reveal their in vivo roles and relative contribution to PDGF regulated developmental processes.  The same platform can be used to search for genes of specific biological significance in a broad array of experimental settings, providing a fast track from gene identification to functional validation.

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