International Mammalian Genome Society

logo18th International Mouse Genome Conference

17-22 October 2004, Seattle, USA


POSTER 33 - SENSITIZED MUTAGENESIS SCREEN FOR MODIFIERS OF THE DELTA/NOTCH PATHWAY IN THE MOUSE

Rubio-Aliaga I, Soewarto D, Wagner S, Przemeck G, Fuchs H, Hrabé de Angelis M

GSF Research Center - Institute of Experimental Genetics, Neuherberg/Munich, Germany

Delta1 encodes a transmembrane protein, which interacts directly with transmembrane proteins of the Notch-family, in a ligand-receptor-like manner. The Delta/Notch signal transduction pathway is well conserved and plays a central role in mediating cell-to-cell communication regulating the determination of various cell-fates during development. Disruption of the Delta1 gene implies alteration of early embryonic development followed by embrionic lethality (Hrabé de Angelis et al., 1997). Moreover, recent studies indicate that the Delta/Notch pathway belongs to a sophisticated system of interactions, which is believed to be involved in a wide range of common diseases including cancer, immunological diseases, infections as well as cardiovascular and neurological diseases. As proven recently, genetic modifier screens, which have been demonstrated to be very powerful in lower organisms, are also feasible in the mouse (Carpinelli et al., 2004). Combining knockout technology and ENU mutagenesis, we are carrying out a sensitized screen with the Delta1 knockout in order to identify enhancers and suppressors of the Delta/Notch pathway. Here we present recent results of this sensitized screen.

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