International Mammalian Genome Society

The 13th International Mouse Genome Conference
October 31-November 3, 1999

Table of Contents * Structure * Bioinformatics * Sequence * Mapping * New Tools * Gene Discovery * Developmental * Mutagenesis * Functional Genomics

F20 Progress in Characterization of a Recessive Lethal Mutant Near the D17Aus9 Locus

Hanna Chao, Sarah Mentzer, Rebecca Bergstrom*, John Schimenti*, and Yun You. Life Sciences Division, Oak Ridge National Laboratory, PO Box 2009, Oak Ridge, Tennessee 37831-8077, USA; *The Jackson Laboratory, Bar Harbor, ME 04609, USA

To systematically identify genes important for mammalian development only a few experimental approaches have been employed so far. One of them is to isolate murine homologues for genes that have been identified in other species, especially Drosophila melanogaster and study their roles during the mouse development. Another approach is to use the promoter or enhancer trap strategy to identify genes important for the mouse development. Characterizing mice carrying spontaneous or induced mutations and positional cloning of genes responsible for the phenotypes may also lead to identification of genes essential for mammalian development. This approach has the potential to discover novel genes that may be missed by other methods. A high throughput method has recently been developed in Dr. John Schimenti's lab at the Jackson Laboratory to induce chromosomal deletions at any region of the mouse genome by radiation in embryonic stem (ES) cells. Lines of mice carrying deletions around the D17Aus9 locus have been generated by this strategy. Deletion analysis of mice carrying a small deletion called D17Aus9df12J showed that an early lethal gene is located near the D17Aus9 locus. Early lethality may mask a later action gene. In order to search for potential later action genes Del(17)T7J, a mutant line carrying a deletion which does not encompass the D17Aus9 locus, but overlaps with the deleted region found in D17Aus9df12J, was used in our deletion analysis. By crossing D17Aus9df12J /+ to Del(17)T7J /+, the heterozygous compound deletion D17Aus9df12J/Del(17)T7J unveiled a new recessive lethal allele distal to the early lethal gene. Current progress in characterization of this lethal mutation will be presented.

[Research currently sponsored by USDOE, under contract DE-AC05-960R22464 with Lockheed Martin Energy Research, Inc.]


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