International Mammalian Genome Society

The 13th International Mouse Genome Conference
October 31-November 3, 1999

Table of Contents * Structure * Bioinformatics * Sequence * Mapping * New Tools * Gene Discovery * Developmental * Mutagenesis * Functional Genomics

F25 An Integrated Genetic and Physical Map Around the Doublefoot Locus on Mouse Chromosome 1

Christopher Hayes1, Mark Harrison1, Gillian Morriss-Kay2, Mary Lyon1, Steve DM Brown1. 1MRC UK Mouse Genome Centre and Mammalian Genetics Unit, Harwell, Didcot, Oxon., OX11 0RD, UK, and 2Dept Human Anatomy and Genetics, University of Oxford, Oxford, OX1 3QX, UK

The spontaneous mouse mutant Doublefoot (Dbf) exhibits polydactyly of all four limbs in association with craniofacial defects. This dominant mutation has been mapped to mouse chromosome 1. Further analysis of meioses from 989 backcross progeny has allowed the construction of a high resolution genetic map of the region, which has narrowed down the mutation to a 0.4cM interval. No suppression of recombination has been observed in this region in the backcross, and karyotype analysis of Dbf animals has indicated that there are no chromosomal rearrangements associated with the mutation. The high resolution genetic map has been used as a platform to initiate a positional cloning strategy for the isolation and characterisation of the Dbf gene.

Using the available MIT markers and STS's derived from the ends of BAC and PAC clones a clone map has been assembled, generating an integrated genetic and physical map around and including the Dbf locus. From this physical map several transcriptional units have been identified within the region. One provocative transcript is currently the subject of whole mount in situ hybridisation and mutation screening in wild type and Dbf mutant embryos. Progress on the development of the transcript map and assessment of candidate genes will be presented.


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