International Mammalian Genome Society

The 13th International Mouse Genome Conference
October 31-November 3, 1999

Table of Contents * Structure * Bioinformatics * Sequence * Mapping * New Tools * Gene Discovery * Developmental * Mutagenesis * Functional Genomics

E39 A Genome-Wide Scan for QTLs Controlling 10-Week Body Weight in a Backcross Population of C57BL/6J and Philippine Wild Mice

Akira Ishikawa, Yoichi Matsuda*, and Takao Namikawa. Laboratory of Animal Genetics, School of Agricultural Sciences, Nagoya University, Nagoya 464-8601, Japan. *Present address: Chromosome Research Unit, Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan

A genome-wide scan for quantitative trait loci (QTLs) controlling body weight at 10 weeks after birth was carried out in the population of 387 intersubspecific backcross mice derived from a cross between C57BL/6J inbred mice and wild mice (M. m. castaneus) captured in Philippines. The wild mice had body weight about 60% lower than C57BL/6J. After adjustment of sex and litter effects, the 89 heaviest and 87 lightest backcross mice were selectively genotyped for fully informative 88 microsatellite markers. All backcross mice were genotyped for a number of markers around the chromosome regions with the peak LOD scores exceeding chromosome-specific 5% threshold levels. The QTL Cartographer program was used for QTL analysis. By interval mapping, we detected a single significant QTL on Chromosome (Chr) 2 in both sexes (LOD=3.1-4.8; 7.6-12.1% of total variance), three suggestive male-specific QTLs on Chrs 11, 13 and X (1.6-2.3; 4.5-6.9%), and three suggestive female-specific QTLs on Chrs 9, 10 and 15 (1.6; 3.8-4.4%). A composite interval mapping analysis confirmed the presence of the four QTLs on Chrs 2, 11, 13 and 15 (2.0-6.1; 4.3-13.5%), and newly located a single significant female-specific QTL on X Chr (4.9; 10.3%) and three suggestive female-specific QTLs on Chrs 9, 11 and 13 (1.7-2.6; 3.5-4.3%), the locations of which were all not identical with those of the above QTLs. All the QTL alleles derived from the wild mice decreased body weight. A two-way analysis of variance revealed a digenic epistatic interaction between the Chr 2 QTL and the background marker locus D12Mit4 on Chr 12 only in males (P=0.000010). Previously, no QTLs for growth and fatness have been mapped at least in this region of the Chr 2 QTL. These results suggested that a wild population may be a repository of genetic resources for novel QTLs.


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