International Mammalian Genome Society


The 13th International Mouse Genome Conference
October 31-November 3, 1999

Table of Contents * Structure * Bioinformatics * Sequence * Mapping * New Tools * Gene Discovery * Developmental * Mutagenesis * Functional Genomics

D22 Analyses of Genomic Sequences from Mouse T-Complex

G.K. Kargul1, P. Waeltz1, P. Ma2, E. Chen2, D. Schlessinger1, R. Nagaraja1, and M.S.H. Ko1. 1Laboratory of Genetics, National Institutes on Aging, Baltimore, MD 21224, USA; 2PE-Applied Biosystems, Foster City, CA 94404, USA

A systematic analysis of 10 Mb of the mouse t-complex region on chromosome 17 has been undertaken to define the content of genes involved in early embryonic development. Mapping and sequence analysis are complicated by the presence of a number of large duplications and inversions in this region. We have initiated the effort to construct an unambiguous STS/BAC based physical map by integrating newly mapped markers (11 Wsu and 16 Ertd markers). We are also identifying corresponding BAC clones for other markers already placed in the t-complex region, making additional STSs from inter-B1 products of individual BACs, and integrating additional genetic data available for the region to complete a physical map and resolve duplicated regions.

Thus far, three BACs spanning approximately 600 kb have been sequenced and are being analyzed. The region, syntenic with human 16p13, contains 28 STSs integrated with 33 overlapping BAC clones. In one BAC of 175 kb, genomic sequence was found that encode the transcripts for Nubp2 (Nucleotide binding protein 2; corresponding to EST D17Wsu11e) and D17Wsu15e. The same sequence also contains the known but previously incompletely mapped Als (which binds and stabilizes the IGF/IGFBP3 complex in serum to extend its half-life), as well as the ortholog of human nucleoside diphosphokinase gene and the 3' end of the ortholog of rat Rsp29 gene. Four additional potential coding regions with CpG islands would yield an overall average of at least one gene every 19.5 kb (a total of 9 genes on this BAC), making this region of the t-complex part of the highest gene density fraction of the genome.

 


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