International Mammalian Genome Society

The 13th International Mouse Genome Conference
October 31-November 3, 1999

Table of Contents * Structure * Bioinformatics * Sequence * Mapping * New Tools * Gene Discovery * Developmental * Mutagenesis * Functional Genomics

E42 QTLs for Alcohol and Sweetener Preference Map to the Same Position on Mouse Distal Chromosome 4

X. Li1, A.A. Bachmanov1, D.R. Reed2, M.G. Tordoff1, R.A. Price2, and G.K. Beauchamp1,2. 1Monell Chemical Senses Center, Philadelphia, PA, 19104; 2University of Pennsylvania, Philadelphia, PA, 19104

In two-bottle preference tests, C57BL/6ByJ (B6) mice consume more ethanol and sweetener solutions than do 129/J (129) mice. There is a positive correlation between ethanol and sweetener intakes among F2 hybrids of these strains. Using the F2 hybrids, we have mapped both traits to the telomeric region of chromosome 4. We created a saturated map of this chromosomal region: D4Mit33 (2.5 cM) D4Mit190 (2.0 cM) D4Mit42 (1.6 cM) D4Mit254 (0.7 cM) D4Mit209 (3.1 cM) D4Mit256 (2.6 cM) D18346. The D18346 sequence tag site (STS) marker was identified from a YAC contig WC-1536 and tested using single strand conformation polymorphism (SSCP) analysis. Peak LOD scores for consumption of ethanol, sucrose and saccharin were all within the D4Mit256 - D18346 interval. This location corresponds to a previously suggested Sac (saccharin preference) locus (Lush et al., Genet. Res. 1995, 66: 167). The position of the Sac locus was confirmed by marker-assisted selection of a 129.B6-Sac congenic strain. Recently, a putative taste receptor gene (TR1) has been mapped to the same chromosomal region (Hoon et al., Cell 1999, 96: 541). To assess the TR1 gene as a candidate for the Sac locus, we are examining TR1 sequence variation between the B6 and 129 strains. This study suggests that the Sac locus determines peripheral taste responsiveness and has a pleiotropic effect on alcohol and sweetener consumption.

Supported by NIH grants R01DC00882, R01AA11028, R01DK44073 and R01DK48095.


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