International Mammalian Genome Society

The 13th International Mouse Genome Conference
October 31-November 3, 1999

Table of Contents * Structure * Bioinformatics * Sequence * Mapping * New Tools * Gene Discovery * Developmental * Mutagenesis * Functional Genomics

E49 A Set of 50 Interspecific Recombinant Congenic Strains Between Mus spretus and C57BL/6: A Tool for the Identification of QTLs and Analysis of Epistasis

Xavier Montagutelli, Stéphanie Voegeling, Marek Szatanik, Jean-Louis Guénet. Unité de Génétique des Mammifères, Institut Pasteur, 25 rue du Docteur Roux - 75724 PARIS CEDEX 15

We have developed a set of over 50 interspecific recombinant congenic strains (IRCS) with C57BL/6 as the recipient strain, and SEG/Pas as the donor strain. According to the breeding scheme, we expected that the contribution of M. spretus genome be around 9% overall. These strains were genotyped after 22 generations of inbreeding (mean value), for over 200 loci. The data obtained show that, although no selection was performed during the inbreeding process, the final percentage of spretus genome found in the strains is only 1.6%, due to counter selection of spretus alleles in the course of inbreeding. These strains are now at a high level of homozygosity, since only 0.5% of the genotypes observed were heterozygous. Taken together, this set covers over 40% of the genome. Each strain carries a small number of short chromosomal fragments of M. spretus origin and therefore represents a multicongenic strain. Because they combine the polymorphism of interspecific crosses and the fertility of intraspecific crosses, these IRCS are a useful and powerful tool to analyze complex traits, perform regional high resolution mapping and study epistatic interactions. Detailed genetic maps will be presented together with genome wide statistics.


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