International Mammalian Genome Society

The 13th International Mouse Genome Conference
October 31-November 3, 1999

Table of Contents * Structure * Bioinformatics * Sequence * Mapping * New Tools * Gene Discovery * Developmental * Mutagenesis * Functional Genomics

I18 Polymorphic Sequences in the mtDNA D-Loop Region of Cheju (Korea) and Tsushima (Japan) Native Horses

Yong Hwan Jung, Gi Ok Kim, Mi Hee Ko, You Sung Oh, Sang Hyun Han, Se Jae Kim, Won Taek Kim, Sun Ju Lee, Kayano Hiroshi* and Moon You Oh. *Faculty of Liberal Arts and Science, Nagasaki University, Japan, Department of Biology, Cheju National University, Cheju 690-756, Korea

The D-loop regions in mitochondrial DNA were amplified from Cheju and Tsushima native horses by polymerase chain reaction (PCR). The oligonucleotide primers used to amplify the equine mtDNA D-loop regions were designed by referring to the sequences of tRNAThr and tRNAPhe in the highly conserved regions of mtDNA common to many other animal species. The total number of bases in the D-loop region were 1,127 bps. The small conserved sequence blocks 1, 2, 1nd 3 that are common to other mammals, were observed. In the middle, there were tandem repeats (19 repeats) of an eight bp equine-specific sequence CACCTGTG. The six horses D-loop regions in this study were appeared to be A/T rich like many other mammals'. In comparison of the sequences among six native horses, twenty five sites of base substitutions (16 transitions and 9 transversions) were found in the region of tRNAPro and in the middle of the D-loop which was highly variable.


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