International Mammalian Genome Society


The 13th International Mouse Genome Conference
October 31-November 3, 1999

Table of Contents * Structure * Bioinformatics * Sequence * Mapping * New Tools * Gene Discovery * Developmental * Mutagenesis * Functional Genomics

E51 Genetic and Physical Mapping of the bronx waltzer Mutation and Investigation of Possible Modifiers

C. S. Nogueira, M. A. Cheong, A. Kelly, T. Bussoli and K. P. Steel. MRC Institute of Hearing Research, University Park, Nottingham, NG7 2RD

Bonx waltzer (bv) is an autosomal recessive mutation that maps to mouse chromosome 5. Mutant mice are deaf and exhibit a waltzing gait. We adopted the positional cloning approach to identify the bv gene. An intraspecific backcross (bv/bv x101/H) F1 x bv/bv comprising over 1000 mice was used to map the bv gene 1.49cM distal to D5Mit25 and 0.37cM proximal to D5Mit209. The EUCIB and BSS interspecific backcross mapping panels were also used to check the location of the markers mapped through the bv backcross in order to locate other markers and genes in the bv region. Recent efforts to isolate the bv gene have focused on physical mapping of the critical region in BACs and YACs, as well as further mapping of STSs, genes and ESTs.

An interspecific backcross between bronx waltzer, on its original genetic background, and Mus castaneus generated homozygous mutant mice with some hearing ability and less severe balance abnormalities than on the original background. The use of a battery of behavioural tests in 241 backcross mice confirmed the presence of modifiers of the bv phenotype introduced from the Mus castaneus genome. In order to map the genetic factor(s) that influence the behaviour and the hearing ability of the bv mutant mice a genome scan is being performed.

 


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