International Mammalian Genome Society


The 13th International Mouse Genome Conference
October 31-November 3, 1999

Table of Contents * Structure * Bioinformatics * Sequence * Mapping * New Tools * Gene Discovery * Developmental * Mutagenesis * Functional Genomics

D24 Genetic, Radiation Hybrid, and Physical Maps of the Region Around the jcpk Locus on Mouse Chromosome 10

Sarah J. Price1, Kathleen E. Tartaglia2, Lorraine Flaherty2, and Elizabeth C. Bryda1. 1Marshall University School of Medicine, Huntington,WV; 2Wadsworth Center, Albany, NY

The jcpk gene on mouse Chromosome 10 causes a severe, early onset form of polycystic kidney disease (PKD) when inherited in an autosomal recessive manner. In previous studies, jcpk has been mapped genetically with high resolution to an approximately 1 cM region on Chromosome 10 flanked by D10Mit115/Tfam and D10Mit173. Recent efforts have focused on physically mapping the jcpk gene by isolating a series of overlapping yeast artificial chromosome (YAC) and bacterial artificial chromosome (BAC) clones from the region around the jcpk gene. The T31 mouse radiation hybrid (RH) panel has also been used to generate a framework map of mouse Chromosome 10 with particular emphasis on markers from the jcpk critical region. The RH map is being used as a quick means of assessing whether new markers, ESTs, and/or genes fall within the jcpk critical region and to assist in orienting the ends of BAC/YAC clones relative to the chromosome. Whenever possible, new markers from the critical region are being mapped genetically in the linkage and radiation hybrid maps as well as physically in the BAC/YAC contig. Comparisons of these maps provide some information about the relationship of the genetic distances versus the physical distances for the region around jcpk.

 


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