International Mammalian Genome Society


The 13th International Mouse Genome Conference
October 31-November 3, 1999

Table of Contents * Structure * Bioinformatics * Sequence * Mapping * New Tools * Gene Discovery * Developmental * Mutagenesis * Functional Genomics

I4 OmniBank: Gene Trapping, Sequence Identification, and Bioinformatics Analysis of 17,000 Genes in Mouse Embryonic Stem Cells

Brian P. Zambrowicz, Glenn A. Friedrich, Eric C. Buxton, Stan L. Lilleberg, Christophe Person, and Arthur T. Sands. Lexicon Genetics, Inc., The Wastelands, Texas

The dramatic increase in sequence information in the form of EST's and genomic sequence has created a gene function gap with the identification of new genes far outpacing the rate at which their function may be identified. Lexicon Genetics has developed a novel method of high-throughput gene trapping with broad applicability in eukaryotic genomes. The ability to create mutations in embryonic stem cells on a large scale by our gene trap mutagenesis technology provides a powerful approach for rapidly determining gene function in a mammalian system. Lexicon's gene trap technology allows for the automated production of sequence tags from the trapped genes with rapid bioinformtics analysis. We have used these techniques to create the world's largest library of ES cells called OmniBankTM that now contains greater than 17,000 trapped genes catalogued by their DNA sequence in a relational database. The OmniBank Demonstration Database is available at www.lexgen.com for functional genomics research use.

 


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