International Mammalian Genome Society


The 13th International Mouse Genome Conference
October 31-November 3, 1999

Table of Contents * Structure * Bioinformatics * Sequence * Mapping * New Tools * Gene Discovery * Developmental * Mutagenesis * Functional Genomics

F53 Isolation of a Novel Cochlear Transcript from the wi Region of Mouse Chromosome 4

H. T. Tsai1, P. Mburu1, R. E. Hardisty1, A. Varela1, A. J. W. Paige1, B. W. Kiernan2, J. Fleming2, M. Rogers2, K. P. Steel2 and S. D. M. Brown1. 1MRC Mouse Genome Centre and Mammalian Genetics Unit, Harwell, Oxon, OX11 0RD, UK; 2MRC Institute of Hearing Research, University Park, Nottingham, NG7 2RD, UK

whirler (wi) mice have a neuroepithelial inner ear defect manifested by deafness and typical shaker-waltzer behaviour (head-tossing and circling). High resolution genetic mapping using interspecific and intraspecific backcrosses has mapped wi close to the Orm1 gene on mouse chromosome 4 (Rogers et al., 1999, Mamm. Genome 10, 513). Using the Orm1 gene as a start-point, a physical map of the wi locus has been constructed. A minimal tiling path of 3 BAC clones completely encompasses the wi non-recombinant region (P. Mburu et al., unpublished data). From the most proximal BAC, a novel transcript has been isolated by cDNA selection. It has been shown to be expressed in kidney, brain, spleen and testis using the OriGene cDNA panel. It is also present in a mouse early post-natal (1-3 DAB) cochlear cDNA library (provided by Guy Richardson, University of Sussex). In situ hybridisation is being carried out to localize this transcript in the inner ear. However, genetic analysis employing the recombinant panel from the interspecific backcross, demonstrates that this gene lies proximal to the closest proximal flanking recombination breakpoint, positioning the transcript just outside the wi non-recombinant region. Therefore, this gene is not a candidate for the whirler mutation but merits further investigation as a novel cochlear gene.

 


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