International Mammalian Genome Society


The 13th International Mouse Genome Conference
October 31-November 3, 1999

Table of Contents * Structure * Bioinformatics * Sequence * Mapping * New Tools * Gene Discovery * Developmental * Mutagenesis * Functional Genomics

D30 Physical Mapping and Sequencing in the Mouse t-complex Region

P. Waeltz, E. Jones, D. Schlessinger, M.S.H. Ko and R. Nagaraja Laboratory of Genetics, National Institute on Aging, NIH, Baltimore, MD 21224

The "t-complex" in the proximal half of mouse chromosome 17, has unusual features, including the presence of many recessive embryonic lethal loci and loci for segregation distortion. It has been demonstrated that 20-30% of wild mice carry the t-haplotype with four large inversions compared to laboratory mouse strains. In addition, there are many small duplications and deletions in the region. During the study of collections of cDNAs from preimplantation and periimplantation stages, we have shown that many ESTs cluster within the t-complex region. A systematic functional study of all the genes responsible for these phenotypes and a detailed characterization of inversions and duplications require that they be identified, mapped and characterized to define their biological role. Towards this goal, we have undertaken an effort to build a STS/BAC based physical map of a 10Mb region of the t-complex and contribute to the sequencing of the difficult-to-map region. Physical mapping was initiated by PCR based screening of mouse BAC libraries using ESTs and cDNAs, ultimately moving to hybridization based screening. To increase the density of STSs and determine the extent of overlap between clones, we generated additional STSs from inter-B1 PCR product sequences and BAC end sequences. This report will give the current status of the physical map and outline the sequencing targets. We also report the sequence analysis of the first three BACs from the ongoing effort, in an accompanying report (Kargul et al., these meetings).

 


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