International Mammalian Genome Society

The 14th International Mouse Genome Conference (2000)

Table of Contents * Genes, Physical Mapping and Sequencing * Gene Identification * Bioinformatics * Common Resources * RIKEN Session * Functional Genomics * Complex Traits * Mutagenesis * Developmental Genetics and Differentiation * Verne Chapman Memorial Lecture I and II

A16. Genetic Analysis of Autosomal Recessive Cataract Gene, lr2, using Intersubspecific F2 mice: Fine Mapping and Exclusion of Several Candidate Genes

Sang Dal Rhee¹, Sungjoo Kim Yoon², Yeong-Gwan Park³, Hyoungnam Lee², Sung-Don Yang¹, Masaaki Okumoto³, Kozaburo Esaki¹, Chang-Woo Song¹, and Sang-Seop Han^1
1Toxicological Research Center, KRICT, Taejon Korea
²Research Institute of Molecular Genetics, Catholic Medical School, Seoul, Korea
³RIAST, Osaka Prefecture University, Osaka, Japan

We previously localized lens rupture 2 gene (lr2) causing cataract to mouse chromosome 14. To refine the position of lr2 gene prior to positional cloning, we have generated intersubspecific F₂ mice between cataract mutant mice, CXSD and Mus musculus molossinus (MSM/Ms or MSM). Using total of 586 progeny, of which 444 mice were normal and 142 were cataractous, we determined the order of microsatellite markers and several candidate genes using combination of restriction fragment length polymorphism(RFLP), simple sequence length polymorphisms (SSLPs) and single strand conformational polymorphisms(SSCPs) analysis. The order from centromere to telomere was followings: D14Mit156- D14Mit88 - D14Mit235 - (D14Mit102, 203, Clu, Raftk) - (D14Mit143, 158, 262) - (D14Mit28, 6.1) - (D14Mit87) - (D14Mit86, 217, 216) - (D14Mit39, 159, Adam7) - (D14Mit89, 237) - D14Mit67 - D14Mit34. Additionally, Blk, Ctsb, and Gja3 were located proximal side of D14Mit88. Homozygosity mapping of 142 F₂ cataractous mice and additive mating experiment revealed the commonly-inherited regions at D14Mit28 and D14Mit87, which contains the region syntenic to human Chr 8p21-22. We concluded that lr2 locus was placed in the region between (D14Mit143, 158, 262) and (D14Mit86, 217, 216), approximately 0.6 cM interval, thus excluding Blk, Ctsb, Gja3, Clu, Raftk, and Adam7 as candidate genes for lr2 gene.