International Mammalian Genome Society

The 14th International Mouse Genome Conference (2000)

Table of Contents * Genes, Physical Mapping and Sequencing * Gene Identification * Bioinformatics * Common Resources * RIKEN Session * Functional Genomics * Complex Traits * Mutagenesis * Developmental Genetics and Differentiation * Verne Chapman Memorial Lecture I and II

A6. UK Mouse Sequencing Consortium: One Year On

MRM Botcherby¹, P Denny³, H Hummerich⁵, S Cross⁴, V van Heyningen⁴, R Straw¹, J Greystrong¹, L Atkinson¹, M Campbell¹, M Collins¹, S Jones¹, N Lawrence¹, D Clarke¹, D Bodle¹, G Hunter¹, C Kimberley¹, P North¹, L Shufflebottom¹, P Weston¹, L Cave-Berry¹, L Mathews², O McCann², T Hubbard², R Durbin², M Cadman³, R McKeone³, C Sellick³, M Strivens³, AM Mallon³, V Proutski³, M Iravani⁵, S McGhee⁴, P Little⁵, I Jackson⁴, J Rogers², RD Campbell¹ and SDM Brown^3
1HGMP Resource Centre, Hinxton Genome Campus, Cambridge CB10 1SB, UK
²Sanger Centre, Hinxton Genome Campus, Cambridge CB10 1SB, UK
³MRC UK Mouse Genome Centre and Mammalian Genetics Unit, Harwell, Oxfordshire, OX11ORD, UK
⁴MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh, EH4 2XU, UK
⁵Imperial College, Exhibition Road, South Kensington, London SW7 2AZ, UK

The UK mouse sequencing consortium was set up last October with the remit of underpinning the UK mouse genomics effort by obtaining 50 Mb of genomic sequence, targeting four regions which have been the subject of genomic and biological investigation in the UK:

The WAGR-homologous region on mouse chromosome 2

The brown deletion complex on mouse chromosome 4

The Del(13)Svea36H chromosome 13 deletion

Dmd-Ar region on chromosome X

The project will also complement the large scale ENU mutagenesis programme underway at Harwell, as obtaining the finished genomic sequence will greatly improve the efficiency of mutation scanning.

The principal interest of the project is the discovery of novel genes and features in the targeted regions. The other main rationale is to construct complete transcript maps with accurate gene models derived from bioinformatic and experimental analysis, ultimately allowing highly efficient mutation scanning for positional cloning of ENU-induced mutations.

The first phase of the project required the building up of maps and minimal tiling paths from the RPCI23 C57BL/6J library (1). This stage is nearing its completion and was carried out by the three mapping groups at HGU, Harwell and Imperial College.

The sequence is placed in the public domain via Ensembl (http://www.ensembl.org/) and a project update will be presented.

1- Osoegawa, K. et al. (2000) Bacterial Artificial Chromosome Libraries for Mouse Sequencing and Functional Analysis. Genome Research 10: 116-128

Project web-site: http://mrcseq.har.mrc.ac.uk