International Mammalian Genome Society

The 14th International Mouse Genome Conference (2000)

Table of Contents * Genes, Physical Mapping and Sequencing * Gene Identification * Bioinformatics * Common Resources * RIKEN Session * Functional Genomics * Complex Traits * Mutagenesis * Developmental Genetics and Differentiation * Verne Chapman Memorial Lecture I and II

B33. High-throughput Genome Wide Search for Mice Imprinted Genes Using Parthenogenote and Androgenote with RIKEN Full-length Mouse cDNA Microarray Analysis.

Yosuke Mizuno^1,3, Yasushi Okazaki¹, Yasuhiro Tomaru¹, Hidenori Kiyosawa¹, Yusuke Sotomaru⁵, Tomohiro Kohno⁵, Hiroshi Amanuma^2,3, Masami Muramatsu¹ and Yoshihide Hayashizakib^1,4.
¹CREST, Japan Science and Technology Corporation (JST), Genome Exploration Research Group, Genomic Sciences Center (GSC), Genome Science Laboratory and
²Molecular Cell Science Laboratory, Riken Tsukuba Life Science Center, the Institute of Physical and Chemical Research (RIKEN), Ibaraki 305-0074, Japan,
³Institute of biological sciences and
⁴Institute of Basic Medical Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibarki, 305-0006, Japan,
⁵Depertment of Animal Science, Tokyo University of Agriculture, Tokyo, Japan

Differentially expressed genes between parthenogenote and androgenote are good candidates for the identification of imprinted genes, which are specifically expressed either from the maternal or the paternal allele. To systematically search differentially expressed genes between parthenogenote and androgenote, we used RIKEN Full-Length mouse cDNA Microarray (17K). (See the abstract of Miki, R. et. al.)

We selected about 25 genes as imprinted candidates. Known imprinted genes, such as IgfII (Insulin-like-growth factor II), Snrpn (small nuclear ribonuclear protein) and Neuronatin are included.

For some of the other genes, we have confirmed these results by RT-PCR.

We have also confirmed the imprinted expressions by analyzing SNPs, which we found in the candidate genes, in two F1 crosses between B6 and Jf1 mice.

We propose a high-throughput screening strategy to systematically search the mice imprinted genes.