International Mammalian Genome Society

The 14th International Mouse Genome Conference (2000)

Table of Contents * Genes, Physical Mapping and Sequencing * Gene Identification * Bioinformatics * Common Resources * RIKEN Session * Functional Genomics * Complex Traits * Mutagenesis * Developmental Genetics and Differentiation * Verne Chapman Memorial Lecture I and II

F10. Analysis of Expression Profiles in Anti-Cancer Drug Sensitive and Resistant Breast Cancer Cells using Mouse Full-Length 20K cDNA Micriarray

Yohei Hamaguchi¹, Takashi Ishikawa¹, Tomoyuki Morita¹, Masako Kamiyama¹, Itaru Nishizuka¹, Shinji Takahashi¹, Yasushi Ichikawa¹, Chikara Kunisaki¹, Shinji Togo¹, Yasushi Okazaki², Yoshihide Hayashizaki², and Hiroshi Shimada^1
1Dept. of Surgery II, Yokohama city university school of medicine, Japan
²The Institute of Physical and Chemical Research, RIKEN Genomic Sciences Center, Japan

We have established RIKEN full-length cDNA microarray containing 20K set of unique mouse genes. Using this microarray system, we examined to identify the target genes and the genetic pathways which could contribute to acquire the resistance against the exposure to an anti-cancer drug, etoposide in breast cancer cell lines of mice. The comparison of the expression profiles between FM3A cells and the etoposide-resistant Fvp^rB350 cells treated with etoposide or paclitaxel reveals that a large number of genes including apoptosis-related genes and other unknown genes were differentially expressed. The analysis of the gene expression relative to drug resistance may lead to the discovery of novel targets for cancer treatments.