International Mammalian Genome Society

The 14th International Mouse Genome Conference (2000)

Table of Contents * Genes, Physical Mapping and Sequencing * Gene Identification * Bioinformatics * Common Resources * RIKEN Session * Functional Genomics * Complex Traits * Mutagenesis * Developmental Genetics and Differentiation * Verne Chapman Memorial Lecture I and II

H11. A Large Scale Mutagenesis Screen for the Identification of Novel Genes Involved in Immunoregulation

Karen Staehling-Hampton, George Carlson^#, Mary Brunkow, Fred Ramsdell and Mark W. Appleby
Celltech Chiroscience, Inc.
1631 220th St. SE, Bothell, WA 98021
#Mclaughlin Research Institute, Great Falls Montana

In order to identify novel genes involved in the regulation of the immune system and in the control of inflammatory responses we have initiated a large-scale ENU screen in the mouse. We have established a series of functional screens designed to examine both in-vivo and in-vitro aspects of inflammation and immune responses in all animals in the screen. We have chosen to focus on the identification of recessive mutations, specifically those that have an effect upon function, reasoning that these will yield novel gene products that play a role in disease situations and thus represent therapeutic targets. We are currently screening G3 animals through these assays. We have also developed a backcross strategy and a microsatellite mapping panel to map the mutations. We will present data on our screening strategy, the phenodeviants that have emerged from the program and our plans for genotyping the mice.