International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)

Table of Contents * Complex Genetics * Developmental Genetics * Gene Annotation * Gene Discovery * Genome Sequencing * Functional Genomics * Mutagenesis * Presentations * Verne Chapman Memorial Lecture

Dr Alexander Bachmanov
Monell Chemical Senses Center
3500 Market Street
Philadelphia PA 19104 USA

Co-Authors:  1)Li X, 1)Li S, 2)Neira M, 1&3)Beauchamp GK, 2)Azen EA
Institutions:   1)Monell Chemical Senses Center, 2)University of Wisconsin, 3)University of Pennsylvania 

An acetylated sugar, sucrose octaacetate (SOA), tastes bitter to humans and has an aversive taste to at least some mice and other animals.  In mice, taste aversion to SOA depends on allelic variation of a single locus, Soa.  Three Soa alleles determine 'taster' (Soaa), 'nontaster'  (Soab) and 'demitaster' (Soac) phenotypes of taste sensitivity to SOA.  Although Soa has been mapped to distal chromosome 6, the limits of the Soa region have not been defined.  In this study, mice from congenic strains SW.B6-Soab, B6.SW-Soaa and C3.SW-Soaa/c, and from an outbred CFW strain were genotyped with polymorphic markers on Chromosome 6.  In the congenic strains, the limits of introgressed donor fragments were determined.  In the outbred mice, linkage disequilibrium and haplotype analyses were conducted.  Positions of the markers were further resolved using radiation hybrid mapping.  The results show that the Soa locus is contained in a ~1-cM (3.3 - 4.9 Mb) region including the Prp locus and probably some of the putative bitter taste receptor genes from the Tas2r (T2R) family.