International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)

Table of Contents * Complex Genetics * Developmental Genetics * Gene Annotation * Gene Discovery * Genome Sequencing * Functional Genomics * Mutagenesis * Presentations * Verne Chapman Memorial Lecture

Dr Shigeharu Wakana
RIKEN GSC
214 Maeda-cho, Totsuka-ku
Yokohama
224-0804
Japan

Co-Authors:  1)Wakana Shigeharu, 1)Masuya Hiroshi, 2)Nakai Y, 1)Suzuki T, 1)Inoue M, 1)Minowa O, 1)Toki H, 3)Koorikawa K, 3)Ishii J, 4)Kida Y, 4)Niinaya N, 5)Kaneko Y, 1)Wakana S, 1)Noda T, 2)Gondo Y,1)Shiroishi T
Institutions:   1)Mouse Functional Genomics Research Group, RIKEN GSC Genomic Sciences Centre, 2)Population and Quantitative Genomics Team, RIKEN, Genomic Sciences Centre 3)Hitachi Software Engineering Co., Ltd., 4)Spoc Inc., 5)Japan Software Management

In the mutagenesis program of RIKEN Genomic Sciences Center (GSC), we have started mutant screens focusing on the late-onset phenotypes, such as tumorigenesis, diabetes, hypertension and neurodegeneration, as well as the early-onset phenotypes that can be screened for 3 months after birth. Rate-limiting steps for gene identification in a large-scale mutagenesis program are accurate phenotyping and high-throughput gene mapping. We are now developing a method of statistical analysis of phenotyping data. In addition, to improve gene mapping, we are exploring the possibility of SNP(s)-base genotyping. In this system, we use allelic discrimination chemistry, TaqMan MGD probes (Applied Biosystems, USA), for detecting SNPs of mouse progeny generated from mapping crosses. The whole system is managed by central database, and all procedures including SNP(s) typing and data transferring to mapping program can be semi-automated, which allows high-throughput gene mapping. We present some mapping data of mutants, which were obtained by this SNP-base assay system in RIKEN GSC mutagenesis program.