International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)

Table of Contents * Complex Genetics * Developmental Genetics * Gene Annotation * Gene Discovery * Genome Sequencing * Functional Genomics * Mutagenesis * Presentations * Verne Chapman Memorial Lecture

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POSTER 250 - ANALYSIS OF AYU8008 MOUSE LINE ESTABLISHED BY EXCHANGEABLE GENE TRAP

Miss Kumiko Yoshinobu
Kumamoto University
2-2-1, Honjo
Kumamoto
860-0811
Japan

Co-Authors:  2)Araki K, 2)Yoshimuta J, 2)Sekimoto T, 3)Suzuki M, 2)Yamamura K, 1)Araki M,
Institutions: 1) Gene Technology Center, Kumamoto University, 2) Division of Developmental Genetics, IMEG, Kumamoto University.  3) CARD, Kumamoto University

We are using an exchangeable gene trap to isolate and characterize novel genes involved in mouse development.   Here, we report the analysis of Ayu8008 mouse line obtained by using gene-trapping vector pU-Hachi.

The beta-geo was expressed in the yolk sac, somite and neural tube at E8.5 and E9.5 embryos.   At E13.5 and E15.5, the beta-geo was expressed in the brain, heart and muscle.   At adult, the expression was detected mainly in the brain, heart, lung, kidney, testis and skin.

The trapped gene was a novel gene and showed similarity to several ESTs. We detected two kinds of transcripts by RT-PCR and expected the transcripts were produced by alternative splicing.

The phenotype of the homozygotes was dependent on the genetic background.   The chimeric mice, whose genetic background is mixed with C57BL/6 and CBA, were backcrossed to C57BL/6.   The homozygotes (F1 x F1) were viable and appeared to be phenotypically normal.   However, the homozygotes (F3 x F3) were showed apparent growth retardation and the homozygotes (F6 x F6) were not viable by 1 week old.